Cardiac Wound Healing Post-Myocardial Infarction: a Novel Method to Target Extracellular Matrix Remodeling in the Left Ventricle

Rogelio Zamilpa, Jianhua Zhang, Ying Ann Chiao, Lisandra E. De Castro Brás, Ganesh V. Halade, Yonggang Ma, Sander O. Hacker, Merry L. Lindsey

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Myocardial infarction (MI) is a leading cause of death worldwide. Permanent ligation of the left anterior descending coronary artery (LAD) is a commonly used surgical model to study post-MI effects in mice. LAD occlusion induces a robust wound healing response that includes extracellular matrix (ECM) remodeling. This chapter provides a detailed guide on the surgical procedure to permanently ligate the LAD. Additionally, we describe a prototype method to enrich cardiac tissue for ECM, which allows one to focus on ECM remodeling in the left ventricle following surgically induced MI in mice.

Original languageAmerican English
Title of host publicationWound Regeneration and Repair. Methods in Molecular Biology (Methods and Protocols)
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

Keywords

  • Cardiac wound healing
  • Decellularization
  • Extracellular matrix
  • Inflammation
  • Matrix metalloproteinases
  • Mice
  • Myocardial infarction

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