Abstract
Myocardial infarction (MI) is a leading cause of death worldwide. Permanent ligation of the left anterior descending coronary artery (LAD) is a commonly used surgical model to study post-MI effects in mice. LAD occlusion induces a robust wound healing response that includes extracellular matrix (ECM) remodeling. This chapter provides a detailed guide on the surgical procedure to permanently ligate the LAD. Additionally, we describe a prototype method to enrich cardiac tissue for ECM, which allows one to focus on ECM remodeling in the left ventricle following surgically induced MI in mice.
Original language | American English |
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Title of host publication | Wound Regeneration and Repair. Methods in Molecular Biology (Methods and Protocols) |
DOIs | |
State | Published - Jan 1 2013 |
Externally published | Yes |
Keywords
- Cardiac wound healing
- Decellularization
- Extracellular matrix
- Inflammation
- Matrix metalloproteinases
- Mice
- Myocardial infarction