Evaluation of Digestion Methods for Analysis of Trace Metals in Mammalian Tissues and NIST 1577c

Grace A. Binder; Rainer Metcalf; Zachary Atlas; Kenyon G. Daniel

doi:10.1016/j.ab.2017.11.016

Evaluation of Digestion Methods for Analysis of Trace Metals in Mammalian Tissues and NIST 1577c

Grace A. Binder, Rainer Metcalf, Zachary Atlas, Kenyon G. Daniel

School of Geosciences

Research output: Contribution to journal › Article › peer-review

Abstract

<a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/digestion" title="Learn more about Digestion from ScienceDirect's AI-generated Topic Pages"> Digestion </a> techniques for ICP analysis have been poorly studied for biological <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/sample" title="Learn more about Sample from ScienceDirect's AI-generated Topic Pages"> samples </a> . This report describes an optimized method for analysis of <a href="https://www.sciencedirect.com/topics/chemistry/trace-metal" title="Learn more about Trace Metal from ScienceDirect's AI-generated Topic Pages"> trace metals </a> that can be used across a variety of sample types. <a href="https://www.sciencedirect.com/topics/chemistry/digestion-methods" title="Learn more about Digestion Methods from ScienceDirect's AI-generated Topic Pages"> Digestion methods </a> were tested and optimized with the analysis of trace metals in cancerous as compared to normal tissue as the end <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/motivation" title="Learn more about Motivation from ScienceDirect's AI-generated Topic Pages"> goal </a> . Anthropological, forensic, oncological and environmental research groups can employ this method reasonably cheaply and safely whilst still being able to compare between laboratories. We examined combined HNO 3 and H 2 O 2 digestion at 170 °C for <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/human" title="Learn more about Human from ScienceDirect's AI-generated Topic Pages"> human </a> , <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/pig" title="Learn more about Pig from ScienceDirect's AI-generated Topic Pages"> porcine </a> and <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/bovine" title="Learn more about Bovine from ScienceDirect's AI-generated Topic Pages"> bovine </a> samples whether they are frozen, fresh or lyophilized <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/powder" title="Learn more about Powder from ScienceDirect's AI-generated Topic Pages"> powder </a> . Little discrepancy is found between <a href="https://www.sciencedirect.com/topics/chemistry/microwave-digestion" title="Learn more about Microwave Digestion from ScienceDirect's AI-generated Topic Pages"> microwave digestion </a> and PFA <a href="https://www.sciencedirect.com/topics/chemistry/poly-tetrafluoroethylene" title="Learn more about Poly(tetrafluoroethylene) from ScienceDirect's AI-generated Topic Pages"> Teflon </a> <a href="https://www.sciencedirect.com/topics/chemistry/pressure-vessel" title="Learn more about Pressure Vessel from ScienceDirect's AI-generated Topic Pages"> pressure vessels </a> . The elements of interest (Cu, Zn, Fe and Ni) yielded consistently higher and more accurate values on standard reference material than samples heated to 75 °C or samples that utilized HNO 3 alone. Use of H 2 SO 4 does not improve <a href="https://www.sciencedirect.com/topics/chemistry/homogeneity" title="Learn more about Homogeneity from ScienceDirect's AI-generated Topic Pages"> homogeneity </a> of the sample and lowers precision during ICP analysis. <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/high-temperature" title="Learn more about High Temperature from ScienceDirect's AI-generated Topic Pages"> High temperature </a> digestions (>165 °C) using a combination of HNO 3 and H 2 O 2 as outlined are proposed as a standard technique for all <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/mammal" title="Learn more about Mammal from ScienceDirect's AI-generated Topic Pages"> mammalian </a> tissues, specifically, human tissues and yield greater than 300% higher values than samples digested at 75 °C regardless of the acid or acid combinations used. The proposed standardized technique is designed to accurately quantify <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/electric-potential" title="Learn more about Electric Potential from ScienceDirect's AI-generated Topic Pages"> potential </a> discrepancies in <a href="https://www.sciencedirect.com/topics/chemistry/metal" title="Learn more about Metal from ScienceDirect's AI-generated Topic Pages"> metal </a> loads between cancerous and healthy tissues and applies to numerous tissue studies requiring quick, effective and safe digestions.

Original language	American English
Journal	Analytical Biochemistry
Volume	543
DOIs	https://doi.org/10.1016/j.ab.2017.11.016
State	Published - Feb 15 2018

Keywords

ICP-OES
Pancreatic cancer
Trace metals
Digestion method
NIST 1577c
ICP-MS

Disciplines

Earth Sciences

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https://doi.org/10.1016/j.ab.2017.11.016

Cite this

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title = "Evaluation of Digestion Methods for Analysis of Trace Metals in Mammalian Tissues and NIST 1577c",

abstract = " Digestion techniques for ICP analysis have been poorly studied for biological samples . This report describes an optimized method for analysis of trace metals that can be used across a variety of sample types. Digestion methods were tested and optimized with the analysis of trace metals in cancerous as compared to normal tissue as the end goal . Anthropological, forensic, oncological and environmental research groups can employ this method reasonably cheaply and safely whilst still being able to compare between laboratories. We examined combined HNO 3 and H 2 O 2 digestion at 170 °C for human , porcine and bovine samples whether they are frozen, fresh or lyophilized powder . Little discrepancy is found between microwave digestion and PFA Teflon pressure vessels . The elements of interest (Cu, Zn, Fe and Ni) yielded consistently higher and more accurate values on standard reference material than samples heated to 75 °C or samples that utilized HNO 3 alone. Use of H 2 SO 4 does not improve homogeneity of the sample and lowers precision during ICP analysis. High temperature digestions (>165 °C) using a combination of HNO 3 and H 2 O 2 as outlined are proposed as a standard technique for all mammalian tissues, specifically, human tissues and yield greater than 300% higher values than samples digested at 75 °C regardless of the acid or acid combinations used. The proposed standardized technique is designed to accurately quantify potential discrepancies in metal loads between cancerous and healthy tissues and applies to numerous tissue studies requiring quick, effective and safe digestions. ",

keywords = "ICP-OES, Pancreatic cancer, Trace metals, Digestion method, NIST 1577c, ICP-MS",

author = "Binder, {Grace A.} and Rainer Metcalf and Zachary Atlas and Daniel, {Kenyon G.}",

year = "2018",

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doi = "10.1016/j.ab.2017.11.016",

language = "American English",

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journal = "Analytical Biochemistry",

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T1 - Evaluation of Digestion Methods for Analysis of Trace Metals in Mammalian Tissues and NIST 1577c

AU - Binder, Grace A.

AU - Metcalf, Rainer

AU - Atlas, Zachary

AU - Daniel, Kenyon G.

PY - 2018/2/15

Y1 - 2018/2/15

N2 - Digestion techniques for ICP analysis have been poorly studied for biological samples . This report describes an optimized method for analysis of trace metals that can be used across a variety of sample types. Digestion methods were tested and optimized with the analysis of trace metals in cancerous as compared to normal tissue as the end goal . Anthropological, forensic, oncological and environmental research groups can employ this method reasonably cheaply and safely whilst still being able to compare between laboratories. We examined combined HNO 3 and H 2 O 2 digestion at 170 °C for human , porcine and bovine samples whether they are frozen, fresh or lyophilized powder . Little discrepancy is found between microwave digestion and PFA Teflon pressure vessels . The elements of interest (Cu, Zn, Fe and Ni) yielded consistently higher and more accurate values on standard reference material than samples heated to 75 °C or samples that utilized HNO 3 alone. Use of H 2 SO 4 does not improve homogeneity of the sample and lowers precision during ICP analysis. High temperature digestions (>165 °C) using a combination of HNO 3 and H 2 O 2 as outlined are proposed as a standard technique for all mammalian tissues, specifically, human tissues and yield greater than 300% higher values than samples digested at 75 °C regardless of the acid or acid combinations used. The proposed standardized technique is designed to accurately quantify potential discrepancies in metal loads between cancerous and healthy tissues and applies to numerous tissue studies requiring quick, effective and safe digestions.

AB - Digestion techniques for ICP analysis have been poorly studied for biological samples . This report describes an optimized method for analysis of trace metals that can be used across a variety of sample types. Digestion methods were tested and optimized with the analysis of trace metals in cancerous as compared to normal tissue as the end goal . Anthropological, forensic, oncological and environmental research groups can employ this method reasonably cheaply and safely whilst still being able to compare between laboratories. We examined combined HNO 3 and H 2 O 2 digestion at 170 °C for human , porcine and bovine samples whether they are frozen, fresh or lyophilized powder . Little discrepancy is found between microwave digestion and PFA Teflon pressure vessels . The elements of interest (Cu, Zn, Fe and Ni) yielded consistently higher and more accurate values on standard reference material than samples heated to 75 °C or samples that utilized HNO 3 alone. Use of H 2 SO 4 does not improve homogeneity of the sample and lowers precision during ICP analysis. High temperature digestions (>165 °C) using a combination of HNO 3 and H 2 O 2 as outlined are proposed as a standard technique for all mammalian tissues, specifically, human tissues and yield greater than 300% higher values than samples digested at 75 °C regardless of the acid or acid combinations used. The proposed standardized technique is designed to accurately quantify potential discrepancies in metal loads between cancerous and healthy tissues and applies to numerous tissue studies requiring quick, effective and safe digestions.

KW - ICP-OES

KW - Pancreatic cancer

KW - Trace metals

KW - Digestion method

KW - NIST 1577c

KW - ICP-MS

UR - https://digitalcommons.usf.edu/geo_facpub/1494

UR - https://doi.org/10.1016/j.ab.2017.11.016

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DO - 10.1016/j.ab.2017.11.016

M3 - Article

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VL - 543

JO - Analytical Biochemistry

JF - Analytical Biochemistry

ER -

Evaluation of Digestion Methods for Analysis of Trace Metals in Mammalian Tissues and NIST 1577c

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