TY - JOUR
T1 - Metagenomic Identification of a Novel Anellovirus in Pacific Harbor Seal (Phoca vitulina richardsii) Lung Samples and its Detection in Samples from Multiple Years
AU - Ng, Terry Fei Fan
AU - Wheeler, Elizabeth
AU - Greig, Denise
AU - Waltzek, Thomas
AU - Gulland, Frances
AU - Breitbart, Mya
PY - 2011/1/1
Y1 - 2011/1/1
N2 - To investigate viral pathogens potentially involved in a mortality event of 21 Pacific harbor seals (Phoca vitulina richardsii) in California in 2000, viral metagenomics was performed directly on lung samples from five individuals. Metagenomics revealed a novel seal anellovirus (SealAV), which clusters phylogenetically with anelloviruses from California sea lions and domestic cats. Using specific PCR, SealAV was identified in lung tissue from two of five animals involved in the 2000 mortality event, as well as one of 20 harbor seal samples examined post-mortem in 2008. The identification of SealAV in multiple years demonstrates that this virus is persistent in the harbor seal population. SealAV is the second anellovirus reported in the lungs of pinnipeds, suggesting that anellovirus infections may be common amongst marine mammals and that more research is needed to understand the roles of these viruses in marine mammal health and disease.
AB - To investigate viral pathogens potentially involved in a mortality event of 21 Pacific harbor seals (Phoca vitulina richardsii) in California in 2000, viral metagenomics was performed directly on lung samples from five individuals. Metagenomics revealed a novel seal anellovirus (SealAV), which clusters phylogenetically with anelloviruses from California sea lions and domestic cats. Using specific PCR, SealAV was identified in lung tissue from two of five animals involved in the 2000 mortality event, as well as one of 20 harbor seal samples examined post-mortem in 2008. The identification of SealAV in multiple years demonstrates that this virus is persistent in the harbor seal population. SealAV is the second anellovirus reported in the lungs of pinnipeds, suggesting that anellovirus infections may be common amongst marine mammals and that more research is needed to understand the roles of these viruses in marine mammal health and disease.
UR - https://digitalcommons.usf.edu/msc_facpub/747
UR - https://doi.org/10.1099/vir.0.029678-0
U2 - 10.1099/vir.0.029678-0
DO - 10.1099/vir.0.029678-0
M3 - Article
C2 - 21402596
VL - 92
JO - Journal of General Virology
JF - Journal of General Virology
ER -