Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis

Md Masudur Rahman; Monira Noor; Kazi Mehetazul Islam; Md Bashir Uddin; Ferdaus M.A Hossain; Mohammad A Zinnah; Mohammad Al Mamun; Mohammad Rafiqul Islam; Seong Kug Eo; Hossam M Ashour

Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis

Md Masudur Rahman, Monira Noor, Kazi Mehetazul Islam, Md Bashir Uddin, Ferdaus M.A Hossain, Mohammad A Zinnah, Mohammad Al Mamun, Mohammad Rafiqul Islam, Seong Kug Eo, Hossam M Ashour

University of South Florida

Research output: Contribution to journal › Article › peer-review

Abstract

Aim: To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. Materials & methods: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. Results: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. Conclusion: Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.

Original language	American English
Journal	Default journal
State	Published - Jan 1 2015

Keywords

bovine tuberculosis, molecular diagnosis, zoonotic transmission

Disciplines

Animal Diseases
Diseases
Medical Microbiology
Medical Sciences

Access to Document

https://login.ezproxy.lib.usf.edu/login?url=https://doi.org/10.2217/FMB.14.139

Cite this

@article{5e5dbd36bd1a4652a29780eafd494a4f,

title = "Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis",

abstract = " Aim: To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. Materials & methods: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. Results: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. Conclusion: Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.",

keywords = "bovine tuberculosis, molecular diagnosis, zoonotic transmission",

author = "Rahman, {Md Masudur} and Monira Noor and Islam, {Kazi Mehetazul} and Uddin, {Md Bashir} and Hossain, {Ferdaus M.A} and Zinnah, {Mohammad A} and {Al Mamun}, Mohammad and Islam, {Mohammad Rafiqul} and Eo, {Seong Kug} and Ashour, {Hossam M}",

note = "Rahman, M. M., Noor, M., Islam, K. M., Uddin, M. B., Hossain, F. M. A., Zinnah, M. A., … Ashour, H. M. (2015). Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis. Future Microbiology, 10(4), 527–535. https://doi.org/10.2217/FMB.14.139",

year = "2015",

month = jan,

day = "1",

language = "American English",

journal = "Default journal",

}

TY - JOUR

T1 - Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis

AU - Rahman, Md Masudur

AU - Noor, Monira

AU - Islam, Kazi Mehetazul

AU - Uddin, Md Bashir

AU - Hossain, Ferdaus M.A

AU - Zinnah, Mohammad A

AU - Al Mamun, Mohammad

AU - Islam, Mohammad Rafiqul

AU - Eo, Seong Kug

AU - Ashour, Hossam M

N1 - Rahman, M. M., Noor, M., Islam, K. M., Uddin, M. B., Hossain, F. M. A., Zinnah, M. A., … Ashour, H. M. (2015). Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis. Future Microbiology, 10(4), 527–535. https://doi.org/10.2217/FMB.14.139

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Aim: To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. Materials & methods: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. Results: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. Conclusion: Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.

AB - Aim: To develop emerging diagnostic technique for bovine tuberculosis and to identify its potential risk factors. Materials & methods: Bacterial genomic DNA was isolated from bovine milk and human sputum samples and subjected to PCR using specific primer pairs. PCR results were validated using bacteriological cultures. Results: PCR amplification of the targeted DNA fragment of Mycobacterium bovis was successful in 12.33% (37/300) of the bovine samples. Interestingly, 500-bp DNA fragment was also amplified in 6.67% (6/90) of the sputum indicating the possibility of zoonotic transmission. Rearing of livestock in household, unpasteurized milk consumption and smoking were identified as potential risk factors. Conclusion: Results of the study may add value to bovine tuberculosis eradication campaigns to achieve the One Health initiative.

KW - bovine tuberculosis, molecular diagnosis, zoonotic transmission

UR - https://digitalcommons.usf.edu/fac_publications/3232

UR - https://login.ezproxy.lib.usf.edu/login?url=https://doi.org/10.2217/FMB.14.139

M3 - Article

JO - Default journal

JF - Default journal

ER -

Molecular diagnosis of bovine tuberculosis in bovine and human samples: implications for zoonosis

Abstract

Keywords

Disciplines

Access to Document

Other files and links

Cite this