TY - JOUR
T1 - Semi-Scavenging Poultry as Carriers of Avian Influenza Genes
AU - Badruzzaman, A T M
AU - Rahman, Md. Masudur
AU - Hasan, Mahmudul
AU - Hossain, Mohammed Kawser
AU - Husna, Asmaul
AU - Hossain, Ferdaus Mohd Altaf
AU - Giasuddin, Mohammed
AU - Uddin, Md Jamal
AU - Islam, Mohammad Rafiqul
AU - Alam, Jahangir
AU - Eo, Seong-Kug
AU - Fasina, Folorunso Oludayo
AU - Ashour, Hossam M
N1 - Badruzzaman, A.T.M.,
Rahman, M.M., Hasan, M., Hossain,
M.K., Husna, A., Hossain, F.M.A.,
Giasuddin, M., Uddin, M.J., Islam,
M.R., Alam, J., Eo, S., Fasina, F.O., & Hossam, H.M. (2022). Semi-Scavenging Poultry as Carriers of Avian Influenza Genes. Life 12 (2), 1-10. https://doi.org/10.3390/life12020320
PY - 2022/1/1
Y1 - 2022/1/1
N2 - Ducks are the natural reservoir of influenza A virus and the central host for the avian influenza virus (AIV) subtype H5N1, which is highly pathogenic. Semi-scavenging domestic ducks allow for the reemergence of new influenza subtypes which could be transmitted to humans. We collected 844 cloacal swabs from semi-scavenging ducks inhabiting seven migratory bird sanctuaries of Bangladesh for the molecular detection of avian influenza genes. We detected the matrix gene (M gene) using real-time RT–PCR (RT–qPCR). Subtyping of the AIV-positive samples was performed by RT–qPCR specific for H5, H7, and H9 genes. Out of 844 samples, 21 (2.488%) were positive for AIV. Subtyping of AIV positive samples (n = 21) revealed that nine samples (42.85%) were positive for the H9 subtype, five (23.80%) were positive for H5, and seven (33.33%) were negative for the three genes (H5, H7, and H9). We detected the same genes after propagating the virus in embryonated chicken eggs from positive samples. Semi-scavenging ducks could act as carriers of pathogenic AIV, including the less pathogenic H9 subtype. This can enhance the pathogenicity of the virus in ducks by reassortment. The large dataset presented in our study from seven areas should trigger further studies on AIV prevalence and ecology.
AB - Ducks are the natural reservoir of influenza A virus and the central host for the avian influenza virus (AIV) subtype H5N1, which is highly pathogenic. Semi-scavenging domestic ducks allow for the reemergence of new influenza subtypes which could be transmitted to humans. We collected 844 cloacal swabs from semi-scavenging ducks inhabiting seven migratory bird sanctuaries of Bangladesh for the molecular detection of avian influenza genes. We detected the matrix gene (M gene) using real-time RT–PCR (RT–qPCR). Subtyping of the AIV-positive samples was performed by RT–qPCR specific for H5, H7, and H9 genes. Out of 844 samples, 21 (2.488%) were positive for AIV. Subtyping of AIV positive samples (n = 21) revealed that nine samples (42.85%) were positive for the H9 subtype, five (23.80%) were positive for H5, and seven (33.33%) were negative for the three genes (H5, H7, and H9). We detected the same genes after propagating the virus in embryonated chicken eggs from positive samples. Semi-scavenging ducks could act as carriers of pathogenic AIV, including the less pathogenic H9 subtype. This can enhance the pathogenicity of the virus in ducks by reassortment. The large dataset presented in our study from seven areas should trigger further studies on AIV prevalence and ecology.
KW - avian influenza, molecular detection, semi-scavenging ducks
UR - https://digitalcommons.usf.edu/fac_publications/4136
UR - https://doi.org/10.3390/life12020320
M3 - Article
JO - Default journal
JF - Default journal
ER -