TY - JOUR
T1 - Surveillance, epidemiological, and virological detection of highly pathogenic H5N1 avian influenza viruses in duck and poultry from Bangladesh
AU - Ansari, Wahedul Karim
AU - Parvej, Md Shafiullah
AU - El Zowalaty, Mohamed E
AU - Jackson, Sally
AU - Bustin, Stephen A
AU - Ibrahim, Adel K
AU - El Zowalaty, Ahmed E
AU - Rahman, Md Tanvir
AU - Zhang, Han
AU - Rahman Khan, Mohammad Ferdousur
AU - Ahamed, Md Mostakin
AU - Rahman, Md Fasiur
AU - Rahman, Marzia
AU - Hussain Nazir, K.H.M. Nazmul
AU - Ahmed, Sultan
AU - Hossen, Md Liakot
AU - Kafi, Md Abdul
AU - Yamage, Mat
AU - Debnath, Nitish C
AU - Ahmed, Graba
AU - Ashour, Hossam M
AU - Rahman, Md Masudur
AU - Noreddin, Ayman
AU - Rahman, Md Bahanur
N1 - Ansari, W. K., Parvej, M. S., El Zowalaty, M. E., Jackson, S., Bustin, S. A., Ibrahim, A. K., … Rahman, M. B. (2016). Surveillance, epidemiological, and virological detection of highly pathogenic H5N1 avian influenza viruses in duck and poultry from Bangladesh. Veterinary Microbiology, 193, 49–59. https://doi.org/10.1016/j.vetmic.2016.07.025
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Avian influenza viruses (AIVs) continue to pose a global threat. Waterfowl are the main reservoir and are responsible for the spillover of AIVs to other hosts. This study was conducted as part of routine surveillance activities in Bangladesh and it reports on the serological and molecular detection of H5N1 AIV subtype. A total of 2169 cloacal and 2191 oropharyngeal swabs as well as 1725 sera samples were collected from live birds including duck and chicken in different locations in Bangladesh between the years of 2013 and 2014. Samples were tested using virus isolation, serological tests and molecular methods of RT-PCR. Influenza A viruses were detected using reverse transcription PCR targeting the virus matrix (M) gene in 41/4360 (0.94%) samples including both cloacal and oropharyngeal swab samples, 31 of which were subtyped as H5N1 using subtype-specific primers. Twenty-one live H5N1 virus isolates were recovered from those 31 samples. Screening of 1,868 blood samples collected from the same birds using H5-specific ELISA identified 545/1603 (34%) positive samples. Disconcertingly, an analysis of 221 serum samples collected from vaccinated layer chicken in four districts revealed that only 18 samples (8.1%) were seropositive for anti H5 antibodies, compared to unvaccinated birds (n = 105), where 8 samples (7.6%) were seropositive. Our result indicates that the vaccination program as currently implemented should be reviewed and updated. In addition, surveillance programs are crucial for monitoring the efficacy of the current poultry vaccinations programs, and to monitor the circulating AIV strains and emergence of AIV subtypes in Bangladesh.
AB - Avian influenza viruses (AIVs) continue to pose a global threat. Waterfowl are the main reservoir and are responsible for the spillover of AIVs to other hosts. This study was conducted as part of routine surveillance activities in Bangladesh and it reports on the serological and molecular detection of H5N1 AIV subtype. A total of 2169 cloacal and 2191 oropharyngeal swabs as well as 1725 sera samples were collected from live birds including duck and chicken in different locations in Bangladesh between the years of 2013 and 2014. Samples were tested using virus isolation, serological tests and molecular methods of RT-PCR. Influenza A viruses were detected using reverse transcription PCR targeting the virus matrix (M) gene in 41/4360 (0.94%) samples including both cloacal and oropharyngeal swab samples, 31 of which were subtyped as H5N1 using subtype-specific primers. Twenty-one live H5N1 virus isolates were recovered from those 31 samples. Screening of 1,868 blood samples collected from the same birds using H5-specific ELISA identified 545/1603 (34%) positive samples. Disconcertingly, an analysis of 221 serum samples collected from vaccinated layer chicken in four districts revealed that only 18 samples (8.1%) were seropositive for anti H5 antibodies, compared to unvaccinated birds (n = 105), where 8 samples (7.6%) were seropositive. Our result indicates that the vaccination program as currently implemented should be reviewed and updated. In addition, surveillance programs are crucial for monitoring the efficacy of the current poultry vaccinations programs, and to monitor the circulating AIV strains and emergence of AIV subtypes in Bangladesh.
KW - Avian Influenza, H5N1, Surveillance, Bangladesh, Duck, Wild birds, Poultry, Live bird markets, Virus, Isolation, Reverse transcription polymerase chain reaction, Influenza A virus
UR - https://digitalcommons.usf.edu/fac_publications/3234
UR - https://login.ezproxy.lib.usf.edu/login?url=https://doi.org/10.1016/j.vetmic.2016.07.025
M3 - Article
JO - Default journal
JF - Default journal
ER -