The Jumbo Squid,em Dosidicus gigas /em (Ommastrephidae), Living in Oxygen Minimum Zones I: Oxygen Consumption Rates and Critical Oxygen Partial Pressures

Lloyd A. Trueblood; Brad A. Seibel

doi:10.1016/j.dsr2.2012.10.004

The Jumbo Squid,em Dosidicus gigas /em (Ommastrephidae), Living in Oxygen Minimum Zones I: Oxygen Consumption Rates and Critical Oxygen Partial Pressures

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Abstract

Dosidicus gigas is a large, metabolically active, epipelagic squid known to undertake diel vertical migrations across a large temperature and oxygen gradient in the Eastern Pacific. Hypoxia is known to cause metabolic suppression in D. gigas . However, the precise oxygen level at which metabolic suppression sets in is unknown. Here we describe a novel ship-board swim tunnel respirometer that was used to measure metabolic rates and critical oxygen partial pressures ( P crit ) for adult squids (2–7 kg). Metabolic rate measurements were validated by comparison to the activity of the Krebs cycle enzyme, citrate synthase, in mantle muscle tissue (2–17 kg). We recorded a mean routine metabolic rate of 5.91 μmol g−1 h−1 at 10 °C and 12.62 μmol g −1 h −1 at 20 °C. A temperature coefficient, Q 10, of 2.1 was calculated. D. gigas had P crits of 1.6 and 3.8 kPa at 10 and 20 °C, respectively. Oxygen consumption rate (MO2) varied with body mass ( M ) according to MO2=11.57 M −0.12±0.03 at 10 °C. Citrate synthase activity varied with body mass according to Y =9.32 M −0.19±0.02.

Original language	American English
Journal	Deep Sea Research Part II: Topical Studies in Oceanography
Volume	95
DOIs	https://doi.org/10.1016/j.dsr2.2012.10.004
State	Published - Jan 1 2013
Externally published	Yes

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https://doi.org/10.1016/j.dsr2.2012.10.004

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title = "The Jumbo Squid,em Dosidicus gigas /em (Ommastrephidae), Living in Oxygen Minimum Zones I: Oxygen Consumption Rates and Critical Oxygen Partial Pressures",

abstract = " Dosidicus gigas is a large, metabolically active, epipelagic squid known to undertake diel vertical migrations across a large temperature and oxygen gradient in the Eastern Pacific. Hypoxia is known to cause metabolic suppression in D. gigas . However, the precise oxygen level at which metabolic suppression sets in is unknown. Here we describe a novel ship-board swim tunnel respirometer that was used to measure metabolic rates and critical oxygen partial pressures ( P crit ) for adult squids (2–7 kg). Metabolic rate measurements were validated by comparison to the activity of the Krebs cycle enzyme, citrate synthase, in mantle muscle tissue (2–17 kg). We recorded a mean routine metabolic rate of 5.91 μmol g−1 h−1 at 10 °C and 12.62 μmol g −1 h −1 at 20 °C. A temperature coefficient, Q 10, of 2.1 was calculated. D. gigas had P crits of 1.6 and 3.8 kPa at 10 and 20 °C, respectively. Oxygen consumption rate (MO2) varied with body mass ( M ) according to MO2=11.57 M −0.12±0.03 at 10 °C. Citrate synthase activity varied with body mass according to Y =9.32 M −0.19±0.02. ",

author = "Trueblood, {Lloyd A.} and Seibel, {Brad A.}",

year = "2013",

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doi = "10.1016/j.dsr2.2012.10.004",

language = "American English",

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journal = "Deep Sea Research Part II: Topical Studies in Oceanography",

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T1 - The Jumbo Squid,em Dosidicus gigas /em (Ommastrephidae), Living in Oxygen Minimum Zones I: Oxygen Consumption Rates and Critical Oxygen Partial Pressures

AU - Trueblood, Lloyd A.

AU - Seibel, Brad A.

PY - 2013/1/1

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N2 - Dosidicus gigas is a large, metabolically active, epipelagic squid known to undertake diel vertical migrations across a large temperature and oxygen gradient in the Eastern Pacific. Hypoxia is known to cause metabolic suppression in D. gigas . However, the precise oxygen level at which metabolic suppression sets in is unknown. Here we describe a novel ship-board swim tunnel respirometer that was used to measure metabolic rates and critical oxygen partial pressures ( P crit ) for adult squids (2–7 kg). Metabolic rate measurements were validated by comparison to the activity of the Krebs cycle enzyme, citrate synthase, in mantle muscle tissue (2–17 kg). We recorded a mean routine metabolic rate of 5.91 μmol g−1 h−1 at 10 °C and 12.62 μmol g −1 h −1 at 20 °C. A temperature coefficient, Q 10, of 2.1 was calculated. D. gigas had P crits of 1.6 and 3.8 kPa at 10 and 20 °C, respectively. Oxygen consumption rate (MO2) varied with body mass ( M ) according to MO2=11.57 M −0.12±0.03 at 10 °C. Citrate synthase activity varied with body mass according to Y =9.32 M −0.19±0.02.

AB - Dosidicus gigas is a large, metabolically active, epipelagic squid known to undertake diel vertical migrations across a large temperature and oxygen gradient in the Eastern Pacific. Hypoxia is known to cause metabolic suppression in D. gigas . However, the precise oxygen level at which metabolic suppression sets in is unknown. Here we describe a novel ship-board swim tunnel respirometer that was used to measure metabolic rates and critical oxygen partial pressures ( P crit ) for adult squids (2–7 kg). Metabolic rate measurements were validated by comparison to the activity of the Krebs cycle enzyme, citrate synthase, in mantle muscle tissue (2–17 kg). We recorded a mean routine metabolic rate of 5.91 μmol g−1 h−1 at 10 °C and 12.62 μmol g −1 h −1 at 20 °C. A temperature coefficient, Q 10, of 2.1 was calculated. D. gigas had P crits of 1.6 and 3.8 kPa at 10 and 20 °C, respectively. Oxygen consumption rate (MO2) varied with body mass ( M ) according to MO2=11.57 M −0.12±0.03 at 10 °C. Citrate synthase activity varied with body mass according to Y =9.32 M −0.19±0.02.

UR - https://digitalcommons.usf.edu/msc_facpub/2356

UR - https://doi.org/10.1016/j.dsr2.2012.10.004

U2 - 10.1016/j.dsr2.2012.10.004

DO - 10.1016/j.dsr2.2012.10.004

M3 - Article

VL - 95

JO - Deep Sea Research Part II: Topical Studies in Oceanography

JF - Deep Sea Research Part II: Topical Studies in Oceanography

ER -

The Jumbo Squid,em Dosidicus gigas /em (Ommastrephidae), Living in Oxygen Minimum Zones I: Oxygen Consumption Rates and Critical Oxygen Partial Pressures

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